Inamura K, Fukase S, Ohta N, Sakata K, Koike Y
Department of Otolaryngology, Yamagata University School of Medicine, Japan.
Acta Otolaryngol Suppl. 1994;511:218-20. doi: 10.3109/00016489409128336.
Anti-neutrophil cytoplasmic antibodies (ANCA), which are present in Wegener's granulomatosis (WG) and other systemic vasculitis, were detected using the immunoperoxidase method rather than the indirect immunofluorescence method. It was possible to distinguish ANCA patterns (c-ANCA, p-ANCA) by the immunoperoxidase method. When neutrophil smears were treated with 0.4% hydrogen peroxide solution, the endogenous peroxidase activity was sufficiently inhibited. ANCA was also detected, using HL-60 cells (human promyelocytic leukemia) and THP-1 cells (human myelocytic leukemia) as substrates.
抗中性粒细胞胞浆抗体（ANCA）存在于韦格纳肉芽肿（WG）和其他系统性血管炎中，采用免疫过氧化物酶法而非间接免疫荧光法进行检测。通过免疫过氧化物酶法可以区分ANCA模式（c-ANCA、p-ANCA）。当中性粒细胞涂片用0.4%过氧化氢溶液处理时，内源性过氧化物酶活性得到充分抑制。也可使用HL-60细胞（人早幼粒细胞白血病细胞）和THP-1细胞（人髓细胞白血病细胞）作为底物来检测ANCA。
