Song J H, Cho H, Park M Y, Kim Y S, Moon H B, Kim Y K, Pai C H
Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Republic of Korea.
Am J Trop Med Hyg. 1994 May;50(5):608-11. doi: 10.4269/ajtmh.1994.50.608.
Salmonella typhi, the etiologic agent of typhoid fever, typically has only a phase-1 flagellar antigen, H1-d (fliC). While most strains of S. typhi have H1-d antigen, 10-20% of Indonesian isolates have been reported to possess H1-j antigen instead. To investigate the presence H1-j strains of S. typhi isolates in Korea, where typhoid fever is still a common infectious problem, we used the polymerase chain reaction (PCR) with a pair of oligonucleotides primers that specifically amplified the flagellin gene of S. typhi. Of 375 isolates of S. typhi tested, only one was shown to possess the H1-j antigen, which was shown by the presence of a 1,269-basepair fragment on agarose gel electrophoresis after the PCR. The isolate with the H1-j antigen was cultured from a Korean-Indonesian man who was already symptomatic in Indonesia and was thought to be an Indonesian strain. Because 375 strains tested in this study were collected from cases with typhoid fever in different regions of Korea during the period from 1986 to 1991, it could be concluded that the mutation rate to j antigen is negligible among S. typhi endemic in Korea.
伤寒杆菌是伤寒热的病原体，通常只有一种1相鞭毛抗原，即H1-d（fliC）。虽然大多数伤寒杆菌菌株具有H1-d抗原，但据报道，10%-20%的印度尼西亚分离株具有H1-j抗原。为了调查韩国伤寒杆菌分离株中H1-j菌株的存在情况，在韩国伤寒热仍是常见感染问题，我们使用一对寡核苷酸引物进行聚合酶链反应（PCR），该引物可特异性扩增伤寒杆菌的鞭毛蛋白基因。在检测的375株伤寒杆菌分离株中，只有一株显示具有H1-j抗原，PCR后在琼脂糖凝胶电泳上出现1269个碱基对的片段表明了这一点。具有H1-j抗原的分离株是从一名韩裔印度尼西亚男子身上培养出来的，他在印度尼西亚时已经出现症状，被认为是一株印度尼西亚菌株。由于本研究中检测的375株菌株是在1986年至1991年期间从韩国不同地区的伤寒热病例中收集的，因此可以得出结论，在韩国流行的伤寒杆菌中，j抗原的突变率可以忽略不计。
