Grossman D A, Witham N D, Burr D H, Lesmana M, Rubin F A, Schoolnik G K, Parsonnet J
Department of Medicine, Stanford University School of Medicine, California 94305-5092.
J Infect Dis. 1995 Jan;171(1):212-6. doi: 10.1093/infdis/171.1.212.
While the H1-d flagellar serotype of Salmonella typhi has been found worldwide, the H1-j serotype occurs only in Indonesia. A cross-sectional survey in Indonesia compared epidemiologic, clinical, and pathogenetic characteristics of these two serotypes. S. typhi isolates were collected from patients with acute typhoid fever in four Indonesian cities. Flagellar serotype was determined by polymerase chain reaction amplification of the fliC locus of the flg gene. Of 321 isolates, 51 (15.9%) were H1-j. Patients with H1-j infection were older than those with H1-d (P < .001). Among 30 patients with known clinical outcomes, H1-j infection was associated with milder clinical illness than H1-d (P = .06). In vitro, H1-j isolates were both less motile on semi-solid agar plates (P = .004) and less invasive of HEp-2 cells (P = .002) than H1-d isolates. The association of decreased severity of illness with decreased motility and invasiveness suggests that flagellar properties are a component of S. typhi's virulence.
虽然伤寒沙门氏菌的H1-d鞭毛血清型在世界各地均有发现，但H1-j血清型仅在印度尼西亚出现。印度尼西亚的一项横断面调查比较了这两种血清型的流行病学、临床和致病特征。从印度尼西亚四个城市的急性伤寒热患者中收集伤寒沙门氏菌分离株。通过聚合酶链反应扩增flg基因的fliC位点来确定鞭毛血清型。在321株分离株中，51株（15.9%）为H1-j。H1-j感染患者比H1-d感染患者年龄更大（P < .001）。在30例已知临床结局的患者中，H1-j感染比H1-d感染的临床疾病症状更轻（P = .06）。在体外，H1-j分离株在半固体琼脂平板上的运动性比H1-d分离株更低（P = .004），对HEp-2细胞的侵袭性也更低（P = .002）。疾病严重程度降低与运动性和侵袭性降低之间的关联表明，鞭毛特性是伤寒沙门氏菌毒力的一个组成部分。
