Frankel G, Newton S M, Schoolnik G K, Stocker B A
Department of Microbiology and Immunology, Stanford University School of Medicine, CA.
EMBO J. 1989 Oct;8(10):3149-52. doi: 10.1002/j.1460-2075.1989.tb08468.x.
Salmonella typhi, the etiologic agent of typhoid fever, typically has only a phase-1 flagellar antigen, d, but some isolates, found only in Indonesia, have antigen j instead, and may have a second flagellar antigen, z66. It appears that intragenic recombination involving a directly repeated 11 bp sequence in the H1-d flagellin gene changed the flagellar antigen to j, by deleting 261 bp in its central, antigenically determinant, part. Sequencing of the hypervariable regions of genes H1-d and H1-j, and hybridization of such genes, after amplification by the polymerase chain reaction, with oligonucleotide probes specific for the deleted segment or for the sequence produced by the recombination confirmed that all the j alleles have the postulated deletion. By applying the polymerase chain reaction to study S. typhi isolates from Jakarta, not previously tested in respect to flagellar antigen, we showed that gene H1-j was nearly as common as H1-d in these isolates.
伤寒沙门氏菌是伤寒热的病原体，通常只有一种1相鞭毛抗原d，但仅在印度尼西亚发现的一些分离株具有抗原j，并且可能有第二种鞭毛抗原z66。似乎涉及H1-d鞭毛蛋白基因中一个直接重复的11bp序列的基因内重组，通过删除其中心抗原决定部分的261bp，将鞭毛抗原变为j。对H1-d和H1-j基因的高变区进行测序，以及在通过聚合酶链反应扩增后，用针对缺失片段或重组产生的序列的寡核苷酸探针对此类基因进行杂交，证实所有j等位基因都有假定的缺失。通过应用聚合酶链反应研究来自雅加达的伤寒沙门氏菌分离株（此前未对其鞭毛抗原进行过检测），我们发现这些分离株中H1-j基因几乎与H1-d基因一样常见。
