用于制备挤压细胞核和染色体压片的装置。
Device to prepare extruded nuclei and chromosome squashes.
作者信息
Berrios M
机构信息
Department of Pharmacological Sciences, School of Medicine, State University of New York at Stony Brook 11794-8651.
出版信息
Biotech Histochem. 1994 Mar;69(2):78-80. doi: 10.3109/10520299409106265.
A horizontal toggle clamp mounted on a rigid base plate makes nuclear extrusion and polytene chromosome squashing simple and reproducible. The base plate has a stage with shallow flat grooves to align the tissue sample directly below the clamp's swivel foot and hold the microscope slide in place during squashing. Appropriate pressure to obtain either extruded nuclei or squash preparations of polytene chromosomes is established empirically by adjusting the clamp's spindle assembly up or down.
安装在刚性基板上的水平肘节夹使核挤出和多线染色体压片变得简单且可重复。基板有一个带有浅平凹槽的载物台，可将组织样本直接对齐在夹子旋转脚下方，并在压片过程中将显微镜载玻片固定到位。通过上下调节夹子的主轴组件，凭经验确定获得挤出核或多线染色体压片制剂所需的适当压力。
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