Anisimov A P
Tsitologiia. 1992;34(11-12):110-2.
A technique to prepare permanent squashed preparations of cell nuclei and chromosomes is proposed. Fix a piece of material on the slide with acetic alcohol (1:3), macerate with a 45% acetic acid, cover with hydrophilic cellophane previously soaked in a 45% acetic acid and then with a cover slip and filter paper to squash finally as it is routinely performed. After that soak off the cover slip with alcohol, post-fix the squashed preparation together with cellophane in alcohol for 5-10 min, unstick the cellophane, pass the preparation through alcohol once again and dry it. The subsequent treatment of the squashed preparation depends on the purpose of investigation. The slide may be tinctured overlaid with photoemulsion for autoradiography, or processed by different ways.
本文提出了一种制备细胞核和染色体永久性压片标本的技术。用醋酸酒精（1:3）将一块材料固定在载玻片上，用45%的醋酸浸渍，盖上预先浸泡在45%醋酸中的亲水性玻璃纸，然后盖上盖玻片和滤纸，最后按常规方法进行压片。之后用酒精浸泡取下盖玻片，将压片标本与玻璃纸一起在酒精中后固定5-10分钟，揭开玻璃纸，将标本再次通过酒精并干燥。压片标本的后续处理取决于研究目的。载玻片可染色后覆盖感光乳剂用于放射自显影，或采用不同方法处理。
