Silva-Rosales L, Lindbo J A, Dougherty W G
Departamento de Ing. Genética, CINVESTAV-IPN, U. Irapuato, México.
Plant Mol Biol. 1994 Mar;24(6):929-39. doi: 10.1007/BF00014446.
Transgenic Nicotiana tabacum cv. Burley 49 plants were generated which expressed a tobacco etch virus (TEV) coat protein (CP) gene construct containing a stop codon positioned at codon 147. This gene construct was expected to produce a TEV CP lacking the carboxy-terminal 118 amino acids of the full-length 264 amino acid CP. TEV CP gene transcripts of the expected size could be detected in transgenic plants but the expected truncated CP could not be detected. Ten independent transgenic lines expressing this form of the TEV CP gene were examined in detail. Two transgenic plant lines were resistant to aphid- or mechanically transmitted TEV and one line was highly resistant. Protoplasts derived from the highly resistant plant line did not support virus replication. The data suggested that the expression of this mutated form of the TEV CP gene could interfere with TEV replication and displayed features associated with RNA-mediated virus resistance.
培育出了转基因烟草品种白肋烟49植株，这些植株表达了一种烟草蚀纹病毒（TEV）外壳蛋白（CP）基因构建体，该构建体在第147密码子处含有一个终止密码子。预期该基因构建体会产生一种TEV CP，其缺少全长264个氨基酸的CP的羧基末端118个氨基酸。在转基因植物中可以检测到预期大小的TEV CP基因转录本，但未检测到预期的截短CP。对十个独立的表达这种形式的TEV CP基因的转基因株系进行了详细检查。两个转基因株系对蚜虫传播或机械传播的TEV具有抗性，一个株系具有高度抗性。来自高度抗性株系的原生质体不支持病毒复制。数据表明，这种突变形式的TEV CP基因的表达可能会干扰TEV复制，并表现出与RNA介导的病毒抗性相关的特征。
