Swaney S, Powers H, Goodwin J, Rosales L S, Dougherty W G
Department of Microbiology, Oregon State University, Corvallis 97331-3804, USA.
Mol Plant Microbe Interact. 1995 Nov-Dec;8(6):1004-11. doi: 10.1094/mpmi-8-1004.
Sense RNA-mediated virus resistance has been described for transgenic plants expressing potyviral capsid protein sequences. This study was undertaken to determine if expression of other viral sequences could induce this type of virus resistance. Plants showing highly resistant or 'recovery' phenotypes were generated by expressing the tobacco etch virus (TEV) 6 kDa/21 kDa reading frames. Expression of translatable or untranslatable versions of this TEV sequence produced resistant lines. Highly resistant and recovery phenotype plants expressing TEV coat protein sequences. High transcription rates with low steady-state levels of the transgene transcript generally correlated with resistance. During recovery and induction of the resistance, RNA and protein steady-state levels decreased 5- to 20-fold, while transcription of the transgene continued at a similar level. A posttranscriptional, cellular system eliminating sequences contained in the transgene transcript and viral genome would be consistent with the results.
对于表达马铃薯Y病毒属病毒衣壳蛋白序列的转基因植物，已报道了有义RNA介导的病毒抗性。本研究旨在确定表达其他病毒序列是否能诱导这种类型的病毒抗性。通过表达烟草蚀纹病毒（TEV）的6 kDa/21 kDa读码框，培育出表现出高度抗性或“恢复”表型的植株。该TEV序列的可翻译或不可翻译版本的表达均产生了抗性株系。表达TEV外壳蛋白序列的植株表现出高度抗性和恢复表型。转基因转录本的高转录率与低稳态水平通常与抗性相关。在抗性恢复和诱导过程中，RNA和蛋白质的稳态水平下降了5至20倍，而转基因转录仍以相似水平持续。一种消除转基因转录本和病毒基因组中所含序列的转录后细胞系统将与这些结果相符。
