Abe T, Early A, Siegert F, Weijer C, Williams J
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertsfordshire, England.
Cell. 1994 Jun 3;77(5):687-99. doi: 10.1016/0092-8674(94)90053-1.
There are cells scattered in the rear, prespore region of the Dictyostelium slug that share many of the properties of the prestalk cells and that are therefore called anterior-like cells (ALCs). By placing the gene encoding a cell surface protein under the control of an ALC-specific promoter and immunologically labeling the living cells, we analyze the movement of ALCs within the slug. There is a posterior to anterior cellular flow, and the ALCs change their movement pattern as they enter the prestalk zone. Prestalk cells are periodically shed from the migrating slug. They must be replaced if the correct ratio of prestalk to prespore cells is to be maintained, and we present evidence for the transdifferentiation of prespore into prestalk cells, with ALCs functioning as intermediates in the transition. The slug has, therefore, a surprisingly dynamic structure, both with respect to cellular differentiation and cell movement.
在盘基网柄菌蛞蝓的后部前孢子区域散布着一些细胞，它们具有许多前柄细胞的特性，因此被称为类前细胞（ALCs）。通过将编码细胞表面蛋白的基因置于ALC特异性启动子的控制下，并对活细胞进行免疫标记，我们分析了ALCs在蛞蝓体内的运动。存在从后向前的细胞流，并且ALCs在进入前柄区时会改变其运动模式。前柄细胞会周期性地从迁移的蛞蝓中脱落。如果要维持前柄细胞与前孢子细胞的正确比例，就必须替换它们，并且我们提供了前孢子细胞转分化为前柄细胞的证据，其中ALCs在转变过程中起中间体的作用。因此，蛞蝓在细胞分化和细胞运动方面都具有惊人的动态结构。
