Hamil K G, Conti M, Shimasaki S, Hall S H
Laboratories for Reproductive Biology, University of North Carolina at Chapel Hill 27599.
Mol Cell Endocrinol. 1994 Mar;99(2):269-77. doi: 10.1016/0303-7207(94)90017-5.
The proto-oncogenes c-fos and c-jun and the related jun-B encode the components of transcription factor, AP-1, a heterodimeric DNA-binding protein that mediates hormone and growth factor-regulated gene expression. In the rat Sertoli cell, FSH rapidly inhibited c-jun gene expression while it stimulated c-fos and jun-B as well as the expression of the more slowly responding, tissue plasminogen activator (tPA) and inhibin alpha-subunit. These early effects of FSH were not inhibited by cycloheximide. Nuclear run-off analyses demonstrated that the FSH-dependent decline in c-jun and increases in c-fos, jun-B, tPA and inhibin alpha-subunit mRNAs were regulated at the transcriptional level. The rates of degradation of c-fos, c-jun and jun-B mRNAs were unaffected by FSH while tPA and inhibin alpha-subunit mRNAs were stabilized. After 8 h of FSH treatment, the transcription of all five genes returned to basal rates. These data demonstrate immediate-early regulation by FSH of the expression of genes encoding components of the transcription factor, AP-1.
原癌基因c-fos和c-jun以及相关的jun-B编码转录因子AP-1的组成成分，AP-1是一种异二聚体DNA结合蛋白，介导激素和生长因子调节的基因表达。在大鼠支持细胞中，促卵泡激素（FSH）迅速抑制c-jun基因表达，同时刺激c-fos和jun-B以及反应较慢的组织纤溶酶原激活物（tPA）和抑制素α亚基的表达。FSH的这些早期作用不受放线菌酮的抑制。核转录分析表明，FSH依赖的c-jun下降以及c-fos、jun-B、tPA和抑制素α亚基mRNA的增加在转录水平受到调节。c-fos、c-jun和jun-B mRNA的降解速率不受FSH影响，而tPA和抑制素α亚基mRNA则被稳定。FSH处理8小时后，所有五个基因的转录恢复到基础水平。这些数据证明FSH对编码转录因子AP-1组成成分的基因表达具有即时早期调节作用。
