Bernardo-Filho M, Cunha M da C, Valsa I de O, de Araujo A C, da Silva F C, da Fonseca A de S
Departamento de Biofisica e Biometria, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Brazil.
Food Chem Toxicol. 1994 May;32(5):477-9. doi: 10.1016/0278-6915(94)90045-0.
Because of the importance of stannous chloride in various fields of human endeavour, the potential genotoxicity of this reducing agent was evaluated by measurement of either the inactivation or the induction of SOS responses in bacteria. Escherichia coli strains used in this work (wild type, uvrA, recA, lexA and uvrA recA) were treated with stannous chloride; the wild type was found to be the most resistant and the double mutant, the most sensitive strain. As these strains present mutations on specific genes for the repair of DNA, stannous chloride would appear to be capable of inducing and/or producing lesions in DNA and, thus, can be considered to be a potential genotoxic agent. This capability was confirmed by the lysogenic induction of E. coli K12 (lambda) (Inductest) and by microscopic observation of E. coli B filamentation.
由于氯化亚锡在人类诸多领域的重要性，通过测量细菌中SOS反应的失活或诱导来评估这种还原剂的潜在遗传毒性。用氯化亚锡处理本研究中使用的大肠杆菌菌株（野生型、uvrA、recA、lexA和uvrA recA）；发现野生型是最具抗性的菌株，而双突变体是最敏感的菌株。由于这些菌株在DNA修复的特定基因上存在突变，氯化亚锡似乎能够在DNA中诱导和/或产生损伤，因此可被视为潜在的遗传毒性剂。通过大肠杆菌K12（λ）的溶原性诱导（诱导试验）和大肠杆菌B丝状化的显微镜观察证实了这种能力。
