放射性药物试剂盒诱导的细胞失活：氯化亚锡的作用

Cellular inactivation induced by a radiopharmaceutical kit: role of stannous chloride.

作者信息

Assis M L, Caceres M R, De Mattos J C, Caldeira-de-Araújo A, Bernardo-Filho M

机构信息

Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, RJ, Brasil.

出版信息

Toxicol Lett. 1998 Nov 12;99(3):199-205. doi: 10.1016/s0378-4274(98)00221-5.

DOI:10.1016/s0378-4274(98)00221-5

PMID:9862286

Abstract

Stannous chloride (SnCl2) has been used in many sectors of human activities such as food manufacturing and in nuclear medicine to produce radiopharmaceuticals labeled with technetium-99m (99mTc). Due to its importance and genotoxic potentiality, we decided to evaluate the biological effect induced by a nuclear medicine kit, which includes SnCl2, in association with glucoheptonic acid (GHA) which is employed for brain and renal scintigraphies. These studies were carried out with the Escherichia coli AB1157 strain and the deoxyribonucleic acid (DNA) plasmid pUC 9.1. The experiments, with different concentrations of SnCl2 and GHA, show an inverse relationship between both agents. When the GHA concentration was increased, the cellular inactivation induced by SnCl2 was reduced, as measured by the number of viable cells. Moreover, GHA protects the DNA molecule against the damage induced by SnCl2.

摘要

氯化亚锡（SnCl₂）已被用于人类活动的许多领域，如食品制造以及核医学中用于生产用锝-99m（⁹⁹ᵐTc）标记的放射性药物。由于其重要性和遗传毒性潜力，我们决定评估一种核医学试剂盒所诱导的生物学效应，该试剂盒包含SnCl₂，并与用于脑和肾闪烁显像的葡庚糖酸（GHA）联合使用。这些研究是使用大肠杆菌AB1157菌株和脱氧核糖核酸（DNA）质粒pUC 9.1进行的。用不同浓度的SnCl₂和GHA进行的实验表明，这两种试剂之间存在反比关系。当GHA浓度增加时，通过活细胞数量测量发现，SnCl₂诱导的细胞失活减少。此外，GHA可保护DNA分子免受SnCl₂诱导的损伤。