关于使用2,4,5-三羟基苯乙胺作为漆酶定量的快速方法。

On the use of 2,4,5-trihydroxyphenethylamine as a rapid method for laccase quantification.

作者信息

Padiglia A, Medda R, Rescigno A, Floris G

机构信息

Istituto di Chimica Biologica, Università di Cagliari.

出版信息

Ital J Biochem. 1994 Jan-Feb;43(1):24-8.

PMID:8206741

Abstract

The sensitivity of the autoxidation of 2,4,5-trihydroxyphenethylamine (6-hydroxydopamine) to increase by laccase was studied. The autoxidation of 6-hydroxydopamine proceeds by a free radical chain reaction involving O2- and produces the corresponding chromogen 6-hydroxydopaminequinone and hydrogen peroxide. The ability of laccase to increase the autoxidation of 6-hydroxydopamine at pH 5.5 has been used as sensitive assay for this enzyme. The results are tabulated and discussed.

摘要

研究了漆酶对2,4,5-三羟基苯乙胺（6-羟基多巴胺）自氧化增加的敏感性。6-羟基多巴胺的自氧化通过涉及超氧阴离子的自由基链式反应进行，并产生相应的色原6-羟基多巴胺醌和过氧化氢。漆酶在pH 5.5时增加6-羟基多巴胺自氧化的能力已被用作该酶的灵敏测定方法。结果列于表中并进行了讨论。