Medda R, Calabrese L, Musci G, Padiglia A, Floris G
Istituto di Chimica Biologica Università di Cagliari, (Italy).
Biochem Mol Biol Int. 1996 Apr;38(4):721-8.
The effects of ceruloplasmin, a blu copper-containing serum glycoprotein, on the oxidation of the neurotoxin 6-hydroxydopamine in several chemical environments were studied. The spontaneous autoxidation of 6-hydroxydopamine proceeded by a free radical chain reaction involving O2-. and produced the corresponding chromogen 6-hydroxydopaminequinone and hydrogen peroxide. The process was accelerated in the presence of horse plasma ceruloplasmin. Yields of hydrogen peroxide in the presence of ceruloplasmin were significantly less than those measured in its absence. This is the first evidence of oxidase activity of ceruloplasmin toward a natural substrate, the 6-hydroxydopamine.
研究了铜蓝蛋白（一种含铜的蓝色血清糖蛋白）在几种化学环境中对神经毒素6-羟基多巴胺氧化的影响。6-羟基多巴胺的自发自氧化通过涉及超氧阴离子（O2-）的自由基链反应进行，并产生相应的色原6-羟基多巴胺醌和过氧化氢。在马血浆铜蓝蛋白存在下，该过程加速。铜蓝蛋白存在时过氧化氢的产量明显低于不存在时测得的产量。这是铜蓝蛋白对天然底物6-羟基多巴胺具有氧化酶活性的首个证据。
