Berger R, Gjedde A, Heck J, Müller E, Krieglstein J, Jensen A
Department of Obstetrics & Gynecology, University of Giessen, Germany.
J Neurochem. 1994 Jul;63(1):271-9. doi: 10.1046/j.1471-4159.1994.63010271.x.
Fetal cerebral metabolism changes during development. The normal fetal metabolic rate must be known to evaluate pathophysiological changes. Therefore, we determined the regional cerebral glucose consumption in the fetal guinea pig. This required the application of the 2-deoxyglucose method to this species. We measured both the transfer coefficients of deoxyglucose and glucose between the maternal arterial plasma and the fetal brain and the lumped constant in chronically prepared undisturbed guinea pig dams using a three-compartment model. Furthermore, the ratio between the initial clearances of deoxyglucose and glucose between the maternal arterial plasma and the fetal brain and the ratio between the phosphorylation coefficients of these substrates in the fetal brain were determined. The total cerebral glucose consumption measured by the deoxyglucose method (10 +/- 1.2 mumol/100 g/min) was similar to that calculated from the glucose concentration and the phosphorylation coefficient of glucose in the cerebrum (10 +/- 0.4 mumol/100 g/min). We conclude that the 2-deoxyglucose method is applicable to the guinea pig, and we further conclude that in the fetal guinea pig cerebral glucose consumption is 10 times lower than that in the adult.
胎儿大脑代谢在发育过程中会发生变化。要评估病理生理变化，必须了解正常胎儿的代谢率。因此，我们测定了豚鼠胎儿大脑各区域的葡萄糖消耗量。这需要将2-脱氧葡萄糖法应用于该物种。我们使用三室模型测量了慢性制备的未受干扰的豚鼠母体动脉血浆与胎儿大脑之间脱氧葡萄糖和葡萄糖的转运系数以及集总常数。此外，还测定了母体动脉血浆与胎儿大脑之间脱氧葡萄糖和葡萄糖的初始清除率之比以及这些底物在胎儿大脑中的磷酸化系数之比。用2-脱氧葡萄糖法测得的大脑葡萄糖总消耗量（10±1.2微摩尔/100克/分钟）与根据大脑中葡萄糖浓度和葡萄糖磷酸化系数计算得出的值（10±0.4微摩尔/100克/分钟）相似。我们得出结论，2-脱氧葡萄糖法适用于豚鼠，并且我们进一步得出结论，豚鼠胎儿大脑的葡萄糖消耗量比成年豚鼠低10倍。
