Berger R, Gjedde A, Rischke R, Müller E, Heck J, Krieglstein J, Jensen A
Department of Obstetrics and Gynecology, Justus-Liebig-Universität Giessen, Germany.
Eur J Obstet Gynecol Reprod Biol. 1996 Jun;66(2):175-8. doi: 10.1016/0301-2115(96)02403-7.
Using the 2-deoxyglucose method, measurements of local cerebral glucose utilization in large fetal animals are very difficult and expensive. To circumvent these problems we recently modified the 2-deoxyglucose method for use in the fetal guinea pig in utero (Berger et al., J Neurochem 1994; 63: 271-279). The present study was designed to measure the rates of local cerebral glucose utilization in fetal guinea pigs at 0.75 of gestation.
After intravenous injection of 14C 2-deoxyglucose into the dams, local cerebral glucose utilization of the fetuses was measured from the time integral of the tracer in the maternal plasma and the autoradiographically determined concentration of the tracer in various parts of the fetal brain.
Fetal cerebral glucose utilization was low as compared to adult animals and varied in different brain structures from 19 +/- 4 to 29 +/- 7 mumol/100 g/min.
This study demonstrates the feasibility to measure local cerebral glucose utilization in undisturbed fetal guinea pigs in utero. We conclude that the low rate of cerebral glucose utilization and its small overall variability may reflect the neurological immaturity of the fetal brain.
运用2-脱氧葡萄糖法来测量大型胎仔动物局部脑葡萄糖利用率非常困难且成本高昂。为规避这些问题，我们最近对2-脱氧葡萄糖法进行了改进，使其可用于子宫内的胎仔豚鼠（伯杰等人，《神经化学杂志》1994年；63卷：271 - 279页）。本研究旨在测量妊娠0.75期胎仔豚鼠局部脑葡萄糖利用率。
向母鼠静脉注射14C 2-脱氧葡萄糖后，根据母体血浆中示踪剂的时间积分以及放射自显影法测定的胎仔脑不同部位示踪剂浓度，来测量胎仔的局部脑葡萄糖利用率。
与成年动物相比，胎仔脑葡萄糖利用率较低，且在不同脑结构中有所差异，范围为19±4至29±7微摩尔/100克/分钟。
本研究证明了在子宫内未受干扰的胎仔豚鼠中测量局部脑葡萄糖利用率的可行性。我们得出结论，脑葡萄糖利用率低及其总体变化小可能反映了胎仔脑的神经不成熟。
