Pollack J, Campbell J M, Potter S M, Erdman J W
Division of Nutritional Sciences, University of Illinois at Urbana-Champaign 61801.
J Nutr. 1994 Jun;124(6):869-73. doi: 10.1093/jn/124.6.869.
Because a yellow color has been observed in the fat pads of Mongolian gerbils fed a nonpurified diet, we designed the current study to determine whether adult male gerbils would absorb beta-carotene intact from a test meal. Thirty-five gerbils (80-90 g) were adapted to the laboratory and fed a standard purified diet free of beta-carotene for 16-19 d. Gerbils were then fed a test meal consisting of 279 nmol of beta-carotene as 10% water-soluble beadlets suspended in 0.5 mL of Ensure. Gerbils (n = 5) were killed at 0, 2, 4, 6, 12, 24 or 72 h after the test meal, blood was obtained by cardiac puncture, and tissues were taken for beta-carotene analysis. No beta-carotene was detected in serum at 0 or 72 h, whereas beta-carotene was present at all other sampling times. Serum beta-carotene peaked at 4 h, at a level of 88 nmol/L. beta-Carotene was detected in the liver of all groups; however, the concentration increased from -34 pmol/g to a maximum concentration of 926 pmol/g at 24 h after the test meal. Other tissues also contained beta-carotene. The results demonstrate that Mongolian gerbils, like ferrets and preruminant calves, absorb beta-carotene intact when beta-carotene is provided at a physiological level in a test meal. This species may be particularly useful for evaluation of the role of antioxidants, such as beta-carotene, in LDL oxidation.
由于在喂食非纯化日粮的蒙古沙鼠脂肪垫中观察到黄色，我们设计了本研究以确定成年雄性沙鼠是否会从测试餐中完整吸收β-胡萝卜素。35只沙鼠（80 - 90克）适应实验室环境，并喂食不含β-胡萝卜素的标准纯化日粮16 - 19天。然后给沙鼠喂食由279纳摩尔β-胡萝卜素组成的测试餐，该β-胡萝卜素以10%的水溶性小丸形式悬浮于0.5毫升的安素中。在测试餐后0、2、4、6、12、24或72小时处死沙鼠（n = 5），通过心脏穿刺采集血液，并取组织进行β-胡萝卜素分析。在0或72小时时血清中未检测到β-胡萝卜素，而在所有其他采样时间均检测到β-胡萝卜素。血清β-胡萝卜素在4小时达到峰值，水平为88纳摩尔/升。在所有组的肝脏中均检测到β-胡萝卜素；然而，其浓度从约34皮摩尔/克增加到测试餐后24小时的最大浓度926皮摩尔/克。其他组织中也含有β-胡萝卜素。结果表明，与雪貂和反刍前小牛一样，当在测试餐中以生理水平提供β-胡萝卜素时，蒙古沙鼠会完整吸收β-胡萝卜素。该物种对于评估抗氧化剂（如β-胡萝卜素）在低密度脂蛋白氧化中的作用可能特别有用。
