Inki P, Stenbäck F, Grenman S, Jalkanen M
Turku Centre for Biotechnology, Finland.
J Pathol. 1994 Apr;172(4):349-55. doi: 10.1002/path.1711720410.
Expression of syndecan-1, a cell surface proteoglycan that binds growth factors and extracellular matrix components, was studied in normal and pathological human uterine cervix using immunohistochemical methods. Normal cervical squamous epithelium showed positive staining for syndecan-1 in all cell layers, except the basal cell layer, whereas endocervical columnar epithelium stained weakly. In non-neoplastic reactive lesions, metaplastic squamous cells were positive for syndecan-1, whereas columnar cells showed weak or negative staining. In cervical condylomas, cells showing koilocytotic atypia were positive for syndecan-1. The progression of cervical intraepithelial neoplasia (CIN) grade I to grade III was associated with reduced syndecan-1 expression and localization of syndecan-1 to more superficial cell layers. In squamous cell carcinomas (SCCs), syndecan-1 expression correlated with histological differentiation, being absent from most poorly differentiated tumours. The results suggest that loss of syndecan-1 from atypical cells is an early event during cervical carcinogenesis and show a close association of syndecan-1 expression with preserved epithelial morphology and differentiation.
利用免疫组织化学方法，研究了正常和病理状态下人类子宫颈中syndecan-1（一种可结合生长因子和细胞外基质成分的细胞表面蛋白聚糖）的表达情况。正常宫颈鳞状上皮在除基底层外的所有细胞层中均显示syndecan-1阳性染色，而宫颈管柱状上皮染色较弱。在非肿瘤性反应性病变中，化生的鳞状细胞syndecan-1呈阳性，而柱状细胞染色较弱或呈阴性。在宫颈尖锐湿疣中，显示挖空细胞异型性的细胞syndecan-1呈阳性。宫颈上皮内瘤变（CIN）I级至III级的进展与syndecan-1表达降低以及syndecan-1定位至更浅表的细胞层有关。在鳞状细胞癌（SCC）中，syndecan-1表达与组织学分化相关，大多数低分化肿瘤中不存在syndecan-1表达。结果表明，非典型细胞中syndecan-1的缺失是宫颈癌发生过程中的早期事件，并显示syndecan-1表达与保留的上皮形态和分化密切相关。
