Kamat Sushant S, Kumar G S, Koshy Ajit V
Department of Oral Pathology and Microbiology, Yogita Dental College and Hospital, Khed, Maharashtra, India.
Dent Res J (Isfahan). 2013 May;10(3):321-7.
Syndecan-1 is abundant in normal tissues and reduced in squamous cell carcinomas. Leukoplakia and oral submucous fibrosis (OSF) are oral pre-cancerous disorders that have potential for malignant transformation. The aim of this study was to evaluate the expression of syndecan-1 in leukoplakia and OSF and to identify its role as a reliable marker for predicting malignant changes.
Expression of syndecan-1 was examined immunohistochemically in 42 cases of oral leukoplakia with or without epithelial dysplasia, 28 cases of OSF and 10 cases of normal oral epithelia as control. Mann-Whiteny 'U' test was used for statistical analysis and the level of significance was fixed at P < 0.05.
Intense syndecan-1 expression was observed in nine cases with normal epithelium. Immunopositivity was lost gradually as the extent of epithelial dysplasia increased. The significant reduction in syndecan-1 expression was observed in leukoplakia as epithelial dysplasia progressed from moderate or severe. Similarly, significant reduction was observed in staining intensities in OSF with dysplasia.
The results reveal that down-regulation of syndecan-1 expression is associated with dysplastic changes in leukoplakia and OSF. Thus syndecan-1 can be considered as marker for predicting malignant changes.
Syndecan-1在正常组织中含量丰富，而在鳞状细胞癌中减少。白斑和口腔黏膜下纤维化（OSF）是具有恶变潜能的口腔癌前病变。本研究的目的是评估Syndecan-1在白斑和OSF中的表达，并确定其作为预测恶变变化的可靠标志物的作用。
采用免疫组织化学方法检测42例伴有或不伴有上皮发育异常的口腔白斑、28例OSF以及10例正常口腔上皮作为对照中Syndecan-1的表达。采用Mann-Whiteny “U”检验进行统计学分析，显著性水平设定为P < 0.05。
在9例正常上皮病例中观察到强烈的Syndecan-1表达。随着上皮发育异常程度的增加，免疫阳性逐渐消失。随着上皮发育异常从中度或重度进展，白斑中Syndecan-1表达显著降低。同样，在伴有发育异常的OSF中染色强度也显著降低。
结果显示，Syndecan-1表达下调与白斑和OSF中的发育异常变化相关。因此，Syndecan-1可被视为预测恶变变化的标志物。
