Lara C, Serra V, Marzo C, Valcuende F, Castells A, Bonilla-Musoles F M
Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Valencia, Spain.
Arch Gynecol Obstet. 1994;255(2):73-9. doi: 10.1007/BF02391801.
Epithelial distribution of filaggrin, a histidine-rich protein related to squamous terminal differentiation, was investigated in 87 cervical biopsies using an avidin-biotin-peroxidase technique with a monoclonal anti-human filaggrin antibody (AKH1). Normal squamous cervical epithelium exhibited a positive homogeneous immunoperoxidase stain in the upper parabasal, intermediate and superficial cell layers. Similar findings were obtained in cervical condylomas, although full-thickness staining was observed in 35.7% of the cases (P < 0.001). Filaggrin expression in CIN was inversely related to the severity of the lesion (P < 0.001). An irregular staining pattern was present in most high-grade CIN. Filaggrin expression was closely connected to the degree of tumour differentiation (P < 0.05) in squamous cell carcinomas of the cervix. Abnormal filaggrin stainings identified a premalignant/malignant cervical squamous lesion with a positive predictive value of 92.3%. Non-squamous epithelia showed lack of filaggrin expression. Filaggrin may therefore be considered a marker of squamous differentiation in both the normal and pathological human uterine cervix.
利用抗人丝聚蛋白单克隆抗体（AKH1）的抗生物素蛋白-生物素-过氧化物酶技术，在87例宫颈活检组织中研究了丝聚蛋白（一种与鳞状终末分化相关的富含组氨酸的蛋白）的上皮分布情况。正常宫颈鳞状上皮在上层基底旁、中层和表层细胞层呈现均匀阳性免疫过氧化物酶染色。宫颈湿疣也有类似发现，不过35.7%的病例观察到全层染色（P<0.001）。丝聚蛋白在宫颈上皮内瘤变（CIN）中的表达与病变严重程度呈负相关（P<0.001）。大多数高级别CIN呈现不规则染色模式。在宫颈鳞状细胞癌中，丝聚蛋白表达与肿瘤分化程度密切相关（P<0.05）。异常的丝聚蛋白染色可识别出宫颈鳞状上皮的癌前/恶性病变，其阳性预测值为92.3%。非鳞状上皮未显示丝聚蛋白表达。因此，丝聚蛋白可被视为正常和病理状态下人类子宫颈鳞状分化的标志物。
