Satoh K, Sato A, Nagahara Y, Kawase Y, Kobayashi T, Kojima K, Tanabe M
Department of Radiology, Kagawa Medical School, Japan.
J Thorac Imaging. 1994 Spring;9(2):112-5. doi: 10.1097/00005382-199421000-00012.
The technique of inflation and fixation of the lung with polyethylene glycol is useful for specimen radiography and radiologic-pathologic correlation, but it is limited by poor histologic staining of the fixed tissue. To improve staining we used formalin to distend and fix the lung before standard fixation with a polyethylene glycol mixture. In this preliminary study, canine and infant lungs, and lungs from three cases of lung cancer were examined. The modified technique provided high-quality staining and satisfactory specimen radiography in all cases except one of the lung cancers; in this case excessive shrinkage occurred and degraded radiographic quality. We conclude that the new method of preparation permits both specimen radiography and high quality staining of the fixed tissue.
用聚乙二醇对肺进行充气和固定的技术对标本射线照相及放射病理对照很有用，但受限于固定组织的组织学染色不佳。为改善染色效果，我们在使用聚乙二醇混合物进行标准固定之前，先用福尔马林使肺膨胀并固定。在这项初步研究中，对犬肺、婴儿肺以及3例肺癌患者的肺进行了检查。除了1例肺癌外，改良技术在所有病例中均提供了高质量的染色和令人满意的标本射线照相；在该病例中出现了过度收缩，降低了射线照相质量。我们得出结论，新的制备方法既允许进行标本射线照相，又能对固定组织进行高质量染色。
