Kobayashi T, Satoh K, Kojima K, Ohkawa M, Tanabe M
Department of Radiology, Kagawa Medical School.
Nihon Igaku Hoshasen Gakkai Zasshi. 1993 Nov 25;53(11):1301-12.
Inflated-fixed lung specimens, prepared using polyethyleneglycol 400, the standard Heitzman method have the disadvantage of bad staining. The authors attempted to improve this short-coming using human autopsied lungs and evaluated both microscopical findings and the degree of specimen inflation. Fifty-five human autopsied whole lungs were examined. Forty-six lungs with cannulation through the main bronchus were distended with 20% formalin, after clamping the cannula for 15 minutes to six days, formalin in lungs was expelled by air at 10 cm H2O pressure, then fixed with fixative-fluid containing polyethyleneglycol 400, 95% ethyl-alcohol, 40% formalin and water mixed in a ratio of 10:5:2:3. In 9 lungs without cannulation, formalin was expelled manually. The degree of distension of 46 of the 55 cannulated specimens was satisfactory (score A: 42 specimens, score B: 4 specimens). In 9 specimens without cannulation, inflation was poor (Score C). Good or excellent staining was obtained in 29 specimens (Score A: 10 specimens, Score B: 19 specimens), but 3 specimens were not be improved (Score C). Both inflation and staining were good in 21 specimens. This new method, requiring cannulation in the main bronchus, inflated-fixation with 20% formalin and expulsion of formalin by air prior to the standard Heitzman method enables both high quality staining and radiographs of the inflated-fixed lung specimen.
使用聚乙二醇400制备的充气固定肺标本，采用标准的海茨曼方法存在染色不佳的缺点。作者试图用人尸检肺来改善这一缺点，并评估微观发现和标本充气程度。检查了55例人尸检全肺。46例通过主支气管插管的肺用20%福尔马林扩张，在夹住插管15分钟至6天后，以10 cm H2O压力用空气排出肺内的福尔马林，然后用含有聚乙二醇400、95%乙醇、40%福尔马林和水按10:5:2:3比例混合的固定液固定。9例未插管的肺，手动排出福尔马林。55例插管标本中有46例的扩张程度令人满意（评分A：42例，评分B：4例）。9例未插管标本的充气情况较差（评分C）。29例标本获得了良好或优秀的染色效果（评分A：10例，评分B：19例），但3例标本染色效果未得到改善（评分C）。21例标本的充气和染色效果均良好。这种新方法需要在主支气管插管，先用20%福尔马林进行充气固定，并在标准海茨曼方法之前用空气排出福尔马林，能够获得高质量的充气固定肺标本染色和X线片。
