Rau W S, Hauenstein K, Mittermayer C, Volk P
Pathol Res Pract. 1980 Dec;170(4):426-32. doi: 10.1016/S0344-0338(80)80047-1.
Radiologic pathologic correlation of lung specimens requires a gas content identical to intravital conditions. Methods of air drying and formalin steam fixation are well known but time consuming. A new, rapid method is presented: the lungs are inflated with compressed air, frozen in liquid nitrogen, and cut into slices. Radiographs taken with soft tissue technique demonstrate structural details down to 200-300 mu. The excellent state of preservation also permits excellent quality of microscopic slides.