Morguet A J, Körber B, Abel B, Hippler H, Wiegand V, Kreuzer H
Department of Cardiology and Pulmonology, Georg August University, Göttingen, Germany.
Lasers Surg Med. 1994;14(3):238-48. doi: 10.1002/lsm.1900140306.
Laser-induced fluorescence may be used to guide laser ablation of atherosclerotic lesions. This study was performed to evaluate arterial autofluorescence spectroscopy in vitro using a single XeCl excimer laser (308 nm) for simultaneous tissue ablation and fluorescence excitation. The laser beam was coupled to a 600-microns silica fiber transmitting 40-50 mJ/mm2 per pulse. The fluorescence radiation emanating retrogradely from the fiber was collected by a concave mirror spectroscopic analysis over a range of 321-657 nm. The arterial media (n = 26), lipid plaques (n = 26), and calcified lesions (n = 27) of aortic specimens from ten human cadavers were investigated in air, saline, and blood. Whereas the spectrum of calcified lesions changed with the surrounding optical medium, the other spectra remained constant. In air and blood, the spectra of arterial media, lipid plaques, and calcified lesions could be differentiated qualitatively and quantitatively (P < 0.0001). In saline, there was no clearcut spectroscopic difference between lipid plaques and calcified lesions. However, normal arterial media and atherosclerotic lesions (lipid plaques plus calcified lesions) could still be discriminated. Thus spectroscopy and plaque ablation can be combined using a single XeCl excimer laser. These encouraging results should stimulate further studies to determine the potential use of this approach to guide laser angioplasty in humans.
激光诱导荧光可用于指导动脉粥样硬化病变的激光消融。本研究旨在使用单台XeCl准分子激光（308nm）在体外评估动脉自体荧光光谱，以同时进行组织消融和荧光激发。激光束耦合到一根600微米的石英光纤，每脉冲传输40 - 50 mJ/mm²。从光纤逆行发出的荧光辐射由一个凹面镜在321 - 657nm范围内进行光谱分析收集。对来自十具人类尸体的主动脉标本的动脉中层（n = 26）、脂质斑块（n = 26）和钙化病变（n = 27）在空气、盐水和血液中进行了研究。虽然钙化病变的光谱随周围光学介质而变化，但其他光谱保持不变。在空气和血液中，动脉中层、脂质斑块和钙化病变的光谱在定性和定量上都可以区分（P < 0.0001）。在盐水中，脂质斑块和钙化病变之间没有明显的光谱差异。然而，正常动脉中层和动脉粥样硬化病变（脂质斑块加钙化病变）仍然可以区分。因此，光谱学和斑块消融可以使用单台XeCl准分子激光相结合。这些令人鼓舞的结果应促使进一步研究，以确定这种方法在指导人类激光血管成形术中的潜在用途。
