Kozurková M, Misúrová E, Kropácová K
Department of Cellular and Molecular Biology, P.J. Safárik University, Kosice, Slovakia.
Neoplasma. 1994;41(2):89-94.
Age-related changes in histones in the liver of normal rats and in rats irradiated with 5.7 Gy gamma rays were examined. Quantitative histone changes in growing and aging rats (from 1 to 28 months of age) were found to be mild only. As they paralleled the DNA changes, the histone/DNA ratio remained stable with age. In total extracted histones there was a decrease in the H1 proportion in older age groups with preceding increase in the H10 proportion. Thirty minutes after irradiation the amount of histones was reduced with age, probably due to an impaired extractability of histones. As the quantitative DNA changes were milder, the histone/DNA ratio decreased in aging liver after irradiation. Similar patterns of changes in proportion of the H1 fraction and H10 subfraction were observed in irradiated and nonirradiated animals, in the former with an earlier onset. Irradiation, therefore, accelerated spontaneous age-related alterations.
研究了正常大鼠肝脏以及接受5.7 Gyγ射线照射的大鼠肝脏中组蛋白的年龄相关变化。结果发现，生长和衰老大鼠（1至28月龄）的组蛋白定量变化仅较为轻微。由于它们与DNA变化平行，组蛋白/DNA比值随年龄保持稳定。在总提取组蛋白中，老年组H1比例下降，而H10比例先升高。照射30分钟后，组蛋白含量随年龄降低，这可能是由于组蛋白的提取能力受损。由于定量DNA变化较轻微，照射后衰老肝脏中的组蛋白/DNA比值降低。在照射和未照射的动物中观察到H1组分和H10亚组分比例的类似变化模式，前者变化出现得更早。因此，照射加速了与年龄相关的自发改变。
