使用聚乙二醇偶联寡核苷酸进行基于杂交的核酸亲和分配
Hybridization-based affinity partitioning of nucleic acids using PEG-coupled oligonucleotides.
作者信息
Jäschke A, Fürste J P, Erdmann V A, Cech D
机构信息
Institut für Organische und Bioorganische Chemie, Humboldt-Universität zu Berlin, Germany.
出版信息
Nucleic Acids Res. 1994 May 25;22(10):1880-4. doi: 10.1093/nar/22.10.1880.
Polyethylene glycol (PEG)-coupled oligonucleotides are partitioned in an aqueous two-phase system PEG/dextran. The affinity of the oligonucleotide for the PEG-rich phase increases proportionally to the length of the coupled PEG polymer. After hybridization, the PEG-coupled oligonucleotide is able to force a complementary nucleic acid strand into the PEG-rich phase. This property can be used for the sequence-specific isolation of nucleic acids through hybridization-based affinity partitioning. The dependence of the partition coefficient in this system on various parameters is described. The application of this principle to multistage chromatographic separations is demonstrated.
聚乙二醇（PEG）偶联的寡核苷酸在聚乙二醇/葡聚糖双水相体系中进行分配。寡核苷酸对富含PEG相的亲和力与偶联的PEG聚合物长度成正比增加。杂交后，PEG偶联的寡核苷酸能够将互补核酸链驱入富含PEG的相中。这一特性可用于通过基于杂交的亲和分配进行核酸的序列特异性分离。描述了该体系中分配系数对各种参数的依赖性。证明了该原理在多级色谱分离中的应用。
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