Zimmerman S B, Murphy L D
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Biopolymers. 1992 Oct;32(10):1365-73. doi: 10.1002/bip.360321010.
The distribution coefficients of single- and double-stranded oligodeoxynucleotides in a PEG 8000/phosphate two-phase system are a function of their chain length. Values of the distribution coefficients are in general agreement with a simple extension of a model for excluded volume effects (the "available volume model") which was applied previously to the distribution of proteins in this system. The current results therefore provide a second set of examples for molecules of very different geometry where the distribution added molecules is controlled by excluded volume interactions between those molecules and the PEG 8000 of the two-phase system.
单链和双链寡脱氧核苷酸在聚乙二醇8000/磷酸盐双相体系中的分配系数是其链长的函数。分配系数的值与先前应用于该体系中蛋白质分配的排除体积效应模型（“可用体积模型”）的简单扩展总体一致。因此，当前结果为几何形状非常不同的分子提供了第二组实例，其中添加分子的分配受这些分子与双相体系的聚乙二醇8000之间的排除体积相互作用控制。
