Foglesong P D, Willis D B
Division of Virology and Molecular Biology, St. Jude Children's Research Center, Memphis, TN.
Virus Genes. 1994 Jan;8(1):47-54. doi: 10.1007/BF01703601.
A preliminary map of the frog virus 3 (FV 3) genome was constructed by hybridization-selection of mRNAs to cloned DNA fragments and translation in reticulocyte lysates. FV 3 mRNAs were hybridized to KpnI, HindIII, and SalI restriction fragments representing the entire FV 3 genome. Two different hybridization conditions were employed in order to discriminate between the hybridization of early and late mRNAs. A total of 43 major and 18 minor early genes and nine major and three minor late genes were mapped. A 24 kb region comprised of the KpnI D, H, and E fragments encodes 8 of the 12 late genes that were mapped.
通过将mRNA与克隆的DNA片段进行杂交选择并在网织红细胞裂解物中进行翻译，构建了蛙病毒3（FV 3）基因组的初步图谱。FV 3 mRNA与代表整个FV 3基因组的KpnI、HindIII和SalI限制性片段进行杂交。采用了两种不同的杂交条件，以区分早期和晚期mRNA的杂交情况。总共定位了43个主要和18个次要的早期基因以及9个主要和3个次要的晚期基因。由KpnI D、H和E片段组成的24 kb区域编码了定位的12个晚期基因中的8个。
