Weinrich S L, Niles E G, Hruby D E
J Virol. 1985 Aug;55(2):450-7. doi: 10.1128/JVI.55.2.450-457.1985.
Among the products of vaccinia virus genes which are expressed late in infection is a major polypeptide (Mr, 65,000) designated L65. Pulse-chase analyses indicated that L65 is not subject to posttranslational cleavage as is the core polypeptide p4b which migrates to a similar position in sodium dodecyl sulfate-polyacrylamide gels. A polypeptide of 65,000 molecular weight produced in reticulocyte lysates programmed with viral mRNA isolated late in infection was identified as L65 by peptide mapping. L65 mRNA was purified by hybridization selection to restriction fragments of the viral genome and translated in vitro. This allowed the gene encoding L65 to be mapped to the rightmost 4.5 kilobase pairs of the HindIII D fragment. Transcriptional mapping of this region of the genome detected a late mRNA which was initiated at 450 base pairs to the right of the HindIII D-A junction, was transcribed in the leftward direction, and was terminated in the nondescript manner typical of vaccinia virus late mRNAs.
在痘苗病毒感染后期表达的基因产物中，有一种主要的多肽（分子量65,000），命名为L65。脉冲追踪分析表明，L65不像核心多肽p4b那样会发生翻译后切割，p4b在十二烷基硫酸钠 - 聚丙烯酰胺凝胶中迁移到相似位置。通过肽图谱分析，在感染后期分离的病毒mRNA编程的网织红细胞裂解物中产生的分子量为65,000的多肽被鉴定为L65。L65 mRNA通过与病毒基因组的限制性片段杂交选择进行纯化，并在体外进行翻译。这使得编码L65的基因被定位到HindIII D片段最右侧的4.5千碱基对处。对该基因组区域的转录图谱分析检测到一种晚期mRNA，它从HindIII D - A连接处右侧450个碱基对处起始，向左转录，并以痘苗病毒晚期mRNA典型的不明确方式终止。
