Zhdan-Pushkina S M, Dronova N V
Mikrobiologiia. 1976 Jan-Feb;45(1):60-6.
The antimicrobial action of polycation proteins from the nuclei--histons with a high content of lysine (f1) and arginine (f3)--on Pseudomonas bacteria was studied. The sensitivity of various species of the same genus, and various strains of the same species, to histons was differential. The differences do not depend on the ability of the cells to liberate into a surrounding medium substances inactivating histons, and on the rate of histon sorption on the cells. Interaction between the bacteria and histons interferes with the permeability of the membranes, so that components of a low molecular weight, including those with the maximum of absorption in UV at 260 nm, are liberated into the surrounding medium. The total RNA of the cells is depolymerized and the activity of RNase increases. No correlation was established between these phenomena and the sensitivity of the bacteria to histons. The duration of the lag period was also studied, as well as the growth rate in the exponential phase and the total yield of the cells; a positive correlation was detected between the duration of the lag period and the sensitivity of the bacteria to histons.
研究了细胞核中的聚阳离子蛋白——富含赖氨酸的组蛋白（f1）和富含精氨酸的组蛋白（f3）对假单胞菌的抗菌作用。同一属的不同种以及同种的不同菌株对组蛋白的敏感性存在差异。这些差异并不取决于细胞向周围培养基中释放使组蛋白失活物质的能力，也不取决于组蛋白在细胞上的吸附速率。细菌与组蛋白之间的相互作用会干扰细胞膜的通透性，从而使低分子量成分，包括在260nm处紫外线吸收最大的那些成分，释放到周围培养基中。细胞的总RNA发生解聚，核糖核酸酶的活性增加。这些现象与细菌对组蛋白的敏感性之间未建立相关性。还研究了延迟期的持续时间、指数生长期的生长速率以及细胞的总产量；检测到延迟期的持续时间与细菌对组蛋白的敏感性之间存在正相关。
