Mizutani T, Hayashi M, Maeda A, Sasaki N, Yamashita T, Kasai N, Namioka S
Department of Laboratory Amimal Science, Faculty of Veterinary Medicine, Hokkaido University Sapporo, Japan.
Adv Exp Med Biol. 1993;342:129-35. doi: 10.1007/978-1-4615-2996-5_21.
Antisense nucleic acids against specific sequences of mouse hepatitis virus (MHV)-RNAs were tested for their inhibitory effects on viral multiplication in mouse DBT cells. An antisense oligonucleotide containing a sequence complementary to leader RNA was synthesized and shown to induce a significant inhibitory effect on the multiplication of MHV-JHM. A vector which expressed the antisense or sense mRNA7 of MHV was transfected into DBT cells. A decreased multiplication of MHV was observed in both cell lines. The transfected cell line which expressed ribozyme against the 5'-end of the MHV genome was established. The rate of inhibition of MHV-multiplication and the quantity of synthesized virus-specific mRNAs in this transfected cell line were the same for both antisense and sense RNA. These results show that antisense nucleic acids might be eligible for use as antiviral agents against MHV multiplication.
针对小鼠肝炎病毒（MHV）-RNA特定序列的反义核酸，在小鼠DBT细胞中测试了它们对病毒增殖的抑制作用。合成了一种含有与前导RNA互补序列的反义寡核苷酸，并显示出对MHV-JHM增殖有显著抑制作用。将表达MHV反义或正义mRNA7的载体转染到DBT细胞中。在这两种细胞系中均观察到MHV增殖减少。建立了表达针对MHV基因组5'端核酶的转染细胞系。在该转染细胞系中，反义RNA和正义RNA对MHV增殖的抑制率以及合成的病毒特异性mRNA的量相同。这些结果表明，反义核酸可能适合用作抗MHV增殖的抗病毒剂。
