针对聚合酶基因的反义RNA对慢性感染小鼠肝炎病毒的细胞中病毒增殖的抑制作用。

Inhibition of viral multiplication in cells chronically infected with mouse hepatitis virus by antisense RNA against the polymerase gene.

作者信息

Maeda A, Hayashi M, Mizutani T, Ishida K, Watanabe T, Namioka S

机构信息

Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

出版信息

J Vet Med Sci. 1995 Jun;57(3):563-5. doi: 10.1292/jvms.57.563.

DOI:10.1292/jvms.57.563

PMID:7548422

Abstract

Recently, we showed that the antisense RNA containing a hammerhead ribozyme sequence against the polymerase gene of mouse hepatitis virus (MHV) inhibited viral multiplication in acute infection [10]. In the present study, we examined the inhibitory effects of an antisense RNA on viral multiplication in chronic MHV infection. In cell line LR-2, in which the 926-nucleotide (nt) antisense RNA containing a ribozyme sequence against the polymerase gene was expressed constitutively at a high level, chronic MHV infection was established through the maintenance of infection over 100 days postinfection (d.p.i.). After 200 d.p.i., no infectious progeny virus was observed in the culture medium of chronically MHV infected LR-2 cells. Our present results showed that the anitsense RNA could also inhibit viral multiplication in chronic MHV infection.

摘要

最近，我们发现含有针对小鼠肝炎病毒（MHV）聚合酶基因的锤头状核酶序列的反义RNA在急性感染中可抑制病毒增殖[10]。在本研究中，我们检测了反义RNA对慢性MHV感染中病毒增殖的抑制作用。在LR-2细胞系中，持续高水平表达含有针对聚合酶基因的核酶序列的926个核苷酸（nt）的反义RNA，通过在感染后100天（d.p.i.）以上维持感染建立慢性MHV感染。在200 d.p.i.后，在慢性感染MHV的LR-2细胞的培养基中未观察到有感染性的子代病毒。我们目前的结果表明，反义RNA在慢性MHV感染中也能抑制病毒增殖。