与前导RNA互补的寡脱氧核苷酸的硫代磷酸酯类似物对小鼠肝炎病毒增殖的抑制作用

Inhibition of mouse hepatitis virus multiplication by phosphorothioate analogues of oligodeoxynucleotides complementary to the leader RNA.

作者信息

Hayashi M, Maeda A, Watanabe T, Hanaki K, Arai S, Nozaki S

机构信息

Department of Veterinary Radiology, Rakuno Gakuen University, Ebetsu, Japan.

出版信息

J Vet Med Sci. 1995 Dec;57(6):1081-3. doi: 10.1292/jvms.57.1081.

DOI:10.1292/jvms.57.1081

PMID:8720052

Abstract

Phosphorothioate oligodeoxynucleotides (PS-oligo) complementary to a leader RNA of mouse hepatitis virus (MHV) were more effective inhibitors of MHV multiplication than natural oligodeoxynucleotides (PO-oligo). Sequence-dependent inhibition of viral multiplication was shown at low concentrations (0.001-0.1 microM) of antisense PS-oligo. Phosphorothioate oligodeoxycytidine, PS-(dC)20 and PS-oligo, which has no significant homology to the MHV sequence, showed inhibitory effects on MHV multiplication at concentrations higher than 0.5 microM. These results showed that PS-oligo was more potent than PO-oligo in inhibition of MHV multiplication and that PS-oligo may inhibit MHV multiplication by two different mechanisms, that is, in sequence-dependent and -independent manners.

摘要

与小鼠肝炎病毒（MHV）前导RNA互补的硫代磷酸寡脱氧核苷酸（PS-oligo）比天然寡脱氧核苷酸（PO-oligo）更有效地抑制MHV增殖。在低浓度（0.001-0.1微摩尔）的反义PS-oligo下显示出对病毒增殖的序列依赖性抑制。硫代磷酸寡脱氧胞苷、PS-(dC)20和与MHV序列无明显同源性的PS-oligo在高于0.5微摩尔的浓度下对MHV增殖显示出抑制作用。这些结果表明，PS-oligo在抑制MHV增殖方面比PO-oligo更有效，并且PS-oligo可能通过两种不同机制抑制MHV增殖，即序列依赖性和非序列依赖性方式。