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水微污染的免疫化学分析

Immunochemical analysis of water micropollution.

作者信息

Aherne G W

机构信息

Biomedical Research Division, School of Biological Sciences, University of Surrey, Guildford, UK.

出版信息

Sci Total Environ. 1993 Aug 7;135(1-3):73-9. doi: 10.1016/0048-9697(93)90277-d.

Abstract

Immunoassays for detecting and measuring organic microcontaminants in water supplies are gaining acceptance and are becoming more widely available. A number of assays for pesticides have been described. Immunoassays especially those utilising enzyme labels are ideally suited for rapid and low cost monitoring of water quality. One of the major attractions of immunoassays for water analyses is the sensitivity that can be achieved without sample preparation. Limits of detection below the EC Maximum Admissible Concentration (0.1 microgram/l) are regularly obtained with acceptable assay performance in terms of accuracy and precision. The selectivity of analysis depends on the specificity of the antibody used. Generally antisera are compound or group specific and unlike conventional analyses only one analyte can be measured at one time. Enhanced luminescence has provided an alternative sensitive and robust endpoint for immunoassays of herbicides and can be adapted to produce semiquantitative results away from the laboratory.

摘要

用于检测和测量供水系统中有机微污染物的免疫分析方法正逐渐得到认可,并且应用越来越广泛。已经描述了多种针对农药的分析方法。免疫分析,尤其是那些使用酶标记的免疫分析,非常适合对水质进行快速且低成本的监测。免疫分析用于水分析的主要吸引力之一是无需样品制备就能实现的灵敏度。在准确度和精密度方面具有可接受的分析性能的情况下,常规可获得低于欧盟最高允许浓度(0.1微克/升)的检测限。分析的选择性取决于所使用抗体的特异性。一般来说,抗血清是针对化合物或基团特异性的,与传统分析不同,一次只能测量一种分析物。增强发光为除草剂的免疫分析提供了一种灵敏且可靠的替代终点,并且可以进行调整以在实验室外产生半定量结果。

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