Connor A, Collins C, Jiang L, McMaster M, Shulman M J
Department of Immunology, University of Toronto, Canada.
Somat Cell Mol Genet. 1993 Jul;19(4):313-20. doi: 10.1007/BF01232744.
In order to expand the experimental material available for genetic and biochemical analyses of the natural immunoglobulin genes, we have isolated a variety of mutant mouse hybridoma cell lines. Some of these mutants have partial or complete deletions of the mu gene. Other mutants have nonsense or frameshift mutations in the exons encoding the variable and the second and third constant region domains of the mu heavy chain. When combined with earlier mutant data, this collection of genotypically and phenotypically tight mutants of known sequence spans most of the 10 kb of the mu gene, providing material for a variety of studies of genetic recombination and mRNA metabolism.
为了扩充可用于天然免疫球蛋白基因遗传和生化分析的实验材料，我们分离出了多种突变小鼠杂交瘤细胞系。其中一些突变体的μ基因存在部分或完全缺失。其他突变体在编码μ重链可变区以及第二和第三恒定区结构域的外显子中存在无义或移码突变。将这些突变数据与早期的突变数据相结合，这一系列已知序列的基因型和表型紧密的突变体涵盖了μ基因10 kb的大部分区域，为各种遗传重组和mRNA代谢研究提供了材料。
