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小鼠免疫球蛋白重链基因座移码突变的后果。

Consequences of frameshift mutations at the immunoglobulin heavy chain locus of the mouse.

作者信息

Baumann B, Potash M J, Köhler G

出版信息

EMBO J. 1985 Feb;4(2):351-9. doi: 10.1002/j.1460-2075.1985.tb03636.x.

DOI:10.1002/j.1460-2075.1985.tb03636.x
PMID:3926482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554193/
Abstract

From an IgM secreting hybridoma line we have isolated 16 spontaneous mutants that produce truncated IgM polypeptides. The size of the mu-mRNAs produced by these mutants is normal, but they express 3- to 100-fold less mu-mRNA and mutant mu-protein than the parental cell line. Nucleotide sequence analysis of cloned mu-genes and/or their mRNAs show frameshift mutations that generate in-phase chain termination codons. The extent of the reduction in mu-mRNA levels depends on the position of the nonsense codon within the gene.

摘要

我们从一个分泌IgM的杂交瘤细胞系中分离出16个自发突变体，这些突变体产生截短的IgM多肽。这些突变体产生的μ - mRNA大小正常，但与亲代细胞系相比，它们表达的μ - mRNA和突变型μ - 蛋白减少了3至100倍。对克隆的μ - 基因和/或其mRNA进行核苷酸序列分析，发现了产生同相链终止密码子的移码突变。μ - mRNA水平降低的程度取决于基因内无义密码子的位置。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4ef/554193/32b70cc5c3df/emboj00267-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4ef/554193/8c05d2e3d4f1/emboj00267-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4ef/554193/fd55628e6a0f/emboj00267-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4ef/554193/a5633fcd9c28/emboj00267-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4ef/554193/32b70cc5c3df/emboj00267-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4ef/554193/8c05d2e3d4f1/emboj00267-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4ef/554193/fd55628e6a0f/emboj00267-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4ef/554193/a5633fcd9c28/emboj00267-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4ef/554193/32b70cc5c3df/emboj00267-0081-a.jpg

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ON THE TOPOGRAPHY OF THE GENETIC FINE STRUCTURE.论遗传精细结构的拓扑学
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Post-transcriptional regulatory networks in immunity.免疫中的转录后调控网络。
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