Kempner E S
Laboratory of Physical Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892.
Trends Biochem Sci. 1993 Jul;18(7):236-9. doi: 10.1016/0968-0004(93)90169-n.
The unusual technique of radiation inactivation has been used to determine the mass of many different macromolecules. Most of the radiation target sizes obtained agree with the known protein structures. However, in several cases the sizes obtained were not easily interpreted since they did not agree with values determined by more conventional methods. Subsequent studies have shown that many of these perplexing radiation target sizes were indeed correct, often revealing unanticipated details about the nature of the systems being studied.
辐射失活这一独特技术已被用于测定许多不同大分子的质量。所获得的大多数辐射靶标大小与已知的蛋白质结构相符。然而,在某些情况下,所获得的大小难以解释,因为它们与通过更传统方法测定的值不一致。后续研究表明,许多这些令人困惑的辐射靶标大小实际上是正确的,常常揭示出有关所研究系统性质的意想不到的细节。