Blanc S, Schmidt I, Kuhl G, Esperandieu P, Lebeurier G, Hull R, Cerutti M, Louis C
Station de Recherches de Pathologie Comparée, INRA-CNRS, Saint Christol-lez-Alès, France.
Virology. 1993 Nov;197(1):283-92. doi: 10.1006/viro.1993.1589.
Cauliflower mosaic virus (CaMV) aphid transmission factor (ATF), produced in a baculovirus expression system, forms paracrystalline structures, as demonstrated by electron microscopic observations. Similar paracrystals were also found in CaMV-infected plants, using immunogold techniques, thus providing the first evidence of such a structure for the CaMV ATF (P18). We demonstrated that the paracrystals can be solubilized to provide an active form of the CaMV ATF which can also be reverted into the paracrystalline aggregated form. This suggests that the paracrystalline structures might act as a source of active CaMV ATF or be the form in which it is stored within the infected cells. A point mutation within the CaMV gene II (which encodes the ATF) leads to the loss of both the paracrystalline structures and the ATF activity. Hence, the paracrystalline structure seems to be a feature of the native (unmodified ) CaMV ATF.
在杆状病毒表达系统中产生的花椰菜花叶病毒（CaMV）蚜虫传播因子（ATF）形成了准晶体结构，这一点通过电子显微镜观察得以证实。利用免疫金技术，在感染CaMV的植物中也发现了类似的准晶体，从而首次为CaMV ATF（P18）提供了这种结构的证据。我们证明，准晶体可以溶解以提供CaMV ATF的活性形式，该活性形式也可以恢复为准晶体聚集形式。这表明准晶体结构可能是活性CaMV ATF的来源，或者是其在受感染细胞内储存的形式。CaMV基因II（编码ATF）内的一个点突变导致准晶体结构和ATF活性丧失。因此，准晶体结构似乎是天然（未修饰）CaMV ATF的一个特征。
