Nakayashiki H, Tsuge S, Kobayashi K, Okuno T, Furusawa I
Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Japan.
J Gen Virol. 1993 Nov;74 ( Pt 11):2469-72. doi: 10.1099/0022-1317-74-11-2469.
The synthesis and accumulation of aphid transmission factor protein (p18) in cauliflower mosaic virus (CaMV)-infected turnip protoplasts were examined in time course and pulse-labelling experiments, comparing an aphid-non-transmissible isolate (CM1841) with an in vitro recombinant aphid-transmissible CaMV (CMBX) generated from the CM1841 isolate. There was little difference in the synthesis and accumulation of p18 between CM1841- and CMBX-infected protoplasts. When the accumulation of p18 in infected leaves was monitored from 3 to 28 days post-symptom emergence (p.e.) by Western blotting, the amount of p18 accumulated in CM1841-infected leaves continuously decreased from 3 days p.e. throughout the experimental period, whereas the amount of p18 in CMBX-infected leaves was lowest at 3 days p.e. and increased thereafter. These results suggested that CM1841 differed from CMBX not in the synthesis of p18 but in the stability of p18 in infected leaves.
通过时间进程实验和脉冲标记实验，研究了感染花椰菜花叶病毒（CaMV）的芜菁原生质体中蚜虫传播因子蛋白（p18）的合成与积累情况，将一种非蚜虫传播分离株（CM1841）与由CM1841分离株产生的体外重组蚜虫传播CaMV（CMBX）进行了比较。在CM1841和CMBX感染的原生质体中，p18的合成与积累几乎没有差异。当通过蛋白质印迹法监测症状出现后3至28天（p.e.）感染叶片中p18的积累情况时，CM1841感染叶片中积累的p18量从症状出现后3天开始在整个实验期间持续下降，而CMBX感染叶片中p18的量在症状出现后3天最低，此后增加。这些结果表明，CM1841与CMBX的不同之处不在于p18的合成，而在于感染叶片中p18的稳定性。
