Black J B, Inoue N, Kite-Powell K, Zaki S, Pellett P E
Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333.
Virus Res. 1993 Jul;29(1):91-8. doi: 10.1016/0168-1702(93)90128-a.
We obtained isolates of human herpesvirus 7 (HHV-7) from 6 of 8 healthy adults by culturing saliva with human umbilical cord blood lymphocytes. These isolates were identified as HHV-7 on the basis of comparisons of restriction endonuclease fragment profiles and hybridization with HHV-7 strain RK DNA. The isolates could be differentiated from HHV-7 strain RK and from each other by their restriction endonuclease fragment profiles. We confirm the finding of frequent isolation of HHV-7 from saliva of healthy adults and report the first dual isolation of human herpesvirus 6 (HHV-6) and HHV-7 from a single saliva specimen. We also describe an in situ hybridization assay that can distinguish between HHV-6 and HHV-7.
我们通过用人脐血淋巴细胞培养唾液，从8名健康成年人中的6人身上获得了人类疱疹病毒7型（HHV - 7）的分离株。根据限制性内切酶片段图谱的比较以及与HHV - 7毒株RK DNA的杂交结果，这些分离株被鉴定为HHV - 7。通过限制性内切酶片段图谱，这些分离株可与HHV - 7毒株RK以及彼此区分开来。我们证实了从健康成年人唾液中频繁分离出HHV - 7这一发现，并报告了首次从单个唾液标本中同时分离出人类疱疹病毒6型（HHV - 6）和HHV - 7。我们还描述了一种可区分HHV - 6和HHV - 7的原位杂交检测方法。
