Foltzer-Jourdainne C, Garaud J C, Nsi-Emvo E, Raul F
Institute National de la Santé et de la Recherche Médicale U. 61, Strasbourg, France.
Am J Physiol. 1993 Sep;265(3 Pt 1):G459-66. doi: 10.1152/ajpgi.1993.265.3.G459.
The regulatory effect of epidermal growth factor (EGF) on the postnatal maturation of sucrase was investigated in the small intestine of suckling and weanling rats. Administration of EGF (0.5 micrograms.g body wt-1.day-1) to suckling rats caused a slight precocious induction of sucrase expression. In weanling rats EGF markedly stimulated sucrase activity; however, at both ages, the effect of hydrocortisone was more potent. When the glucocorticoid antagonist RU-38486 was administered to sucklings, the precocious induction of sucrase activity by hydrocortisone was inhibited by 80%. However, RU-38486 or adrenalectomy did not prevent the inductive effect of EGF, indicating that EGF acts in a glucocorticoid-independent manner. EGF also potentiated the effect of hydrocortisone and dietary sucrose on the precocious induction of sucrase activity in the sucklings. At weaning, administration of an antiserum specific to rat EGF significantly decreased sucrase activity. This study shows the involvement of EGF in the postnatal maturation of intestinal sucrase in the rat.
在哺乳和断奶大鼠的小肠中研究了表皮生长因子（EGF）对蔗糖酶出生后成熟的调节作用。给哺乳大鼠注射EGF（0.5微克·克体重-1·天-1）会导致蔗糖酶表达略有早熟诱导。在断奶大鼠中，EGF显著刺激蔗糖酶活性；然而，在两个年龄段，氢化可的松的作用更强。当给哺乳大鼠注射糖皮质激素拮抗剂RU-38486时，氢化可的松对蔗糖酶活性的早熟诱导被抑制了80%。然而，RU-38486或肾上腺切除术并不能阻止EGF的诱导作用，这表明EGF以不依赖糖皮质激素的方式发挥作用。EGF还增强了氢化可的松和膳食蔗糖对哺乳大鼠蔗糖酶活性早熟诱导的作用。在断奶时，注射针对大鼠EGF的特异性抗血清会显著降低蔗糖酶活性。这项研究表明EGF参与了大鼠肠道蔗糖酶的出生后成熟过程。
