Maekura R
Toneyama National Hospital, Osaka, Japan.
Kekkaku. 1992 Dec;67(12):775-9.
Detection of IgG antibodies against purified cord factor (trehalose-6, 6'-dimycolate) prepared from Mycobacterium tuberculosis H37Rv was carried out by the method of enzyme-linked immunosorbent assay (ELISA) and its diagnostic usefulness was also evaluated in this study. Sera from 65 patients with active pulmonary tuberculosis, 58 patients with inactive pulmonary tuberculosis, 36 patients with diseases other than tuberculosis and 66 healthy adults were examined. Patients with active pulmonary tuberculosis showed significantly higher titers of IgG antibodies against cord factor than other groups (p < 0.001). Patients with inactive pulmonary tuberculosis also showed significantly higher titers of IgG antibodies against cord factor than patients with diseases other than tuberculosis and healthy adults (p < 0.001). An antibody titers of greater than 0.29 were established as a positive ELISA test. For patients with active pulmonary tuberculosis, the ELISA had a sensitivity of 85% and a specificity of 96%. From these results, it is concluded that the detection of IgG antibodies against cord factor is useful for the serodiagnosis of active or inactive pulmonary tuberculosis.
采用酶联免疫吸附测定法（ELISA）检测针对从结核分枝杆菌H37Rv制备的纯化索状因子（海藻糖-6,6'-二霉菌酸酯）的IgG抗体，并在本研究中评估其诊断效用。检测了65例活动性肺结核患者、58例非活动性肺结核患者、36例非结核疾病患者和66例健康成年人的血清。活动性肺结核患者针对索状因子的IgG抗体滴度显著高于其他组（p<0.001）。非活动性肺结核患者针对索状因子的IgG抗体滴度也显著高于非结核疾病患者和健康成年人（p<0.001）。ELISA检测阳性判定抗体滴度大于0.29。对于活动性肺结核患者，ELISA的敏感性为85%，特异性为96%。从这些结果得出结论，检测针对索状因子的IgG抗体对活动性或非活动性肺结核的血清学诊断有用。
