Matson R S, Kimura T
Biochim Biophys Acta. 1976 Aug 2;442(1):76-87. doi: 10.1016/0005-2787(76)90178-7.
Analysis of ferredoxin content in cultures of Euglena gracilis grown in the presence of selective antibiotic inhibitors of protein synthesis resulted in the following conclusions: 1. Ferredoxin is synthesized from cytoplasmic (80s-type) ribosomes; cycloheximide, a potent inhibitor of 80s translation completely abolished the synthesis, while the inhibitors of 70s translation chloramphenicol and erythromycin were not effective. In addition, ferredoxin was detected in a streptomycin-bleached mutant that lacks the chloroplast structure and chloroplast DNA. 2. Ferredoxin's transcript is presumably of nuclear origin; rifampicin, an inhibitor of chloroplast DNA-dependent RNA polymerase did not inhibit synthesis, while the streptomycin-bleached mutant continued to synthesize ferredoxin without chloroplast DNA.
在存在蛋白质合成的选择性抗生素抑制剂的情况下，对纤细裸藻培养物中的铁氧化还原蛋白含量进行分析，得出以下结论：1. 铁氧化还原蛋白由细胞质（80s型）核糖体合成；环己酰亚胺是80s翻译的有效抑制剂，它能完全消除铁氧化还原蛋白的合成，而70s翻译抑制剂氯霉素和红霉素则无效。此外，在缺乏叶绿体结构和叶绿体DNA的链霉素漂白突变体中也检测到了铁氧化还原蛋白。2. 铁氧化还原蛋白的转录本可能起源于细胞核；利福平是叶绿体DNA依赖性RNA聚合酶的抑制剂，它并不抑制铁氧化还原蛋白的合成，而链霉素漂白突变体在没有叶绿体DNA的情况下仍继续合成铁氧化还原蛋白。
