Perl M
Biochem J. 1972 Dec;130(3):813-8. doi: 10.1042/bj1300813.
Cycloheximide at concentrations of 0.1-100mum stimulated chlorophyll synthesis when dark-grown cells of Euglena were illuminated. Chloramphenicol (1-4mm) inhibited chlorophyll synthesis. The effect of cycloheximide on the incorporation of [(14)C]leucine into material insoluble in trichloroacetic acid, and its failure to affect the incorporation of [(32)P]orthophosphate into such material in short incubations, are interpreted as evidence that cycloheximide specifically inhibits protein synthesis by 80S ribosomes. Since the inhibitory effect of chloramphenicol on chlorophyll synthesis is counteracted by the presence of cycloheximide, it is suggested that chlorophyll synthesis is subject to control by a cytoplasmic repressor synthesized on 80S ribosomes, and to a de-repressor synthesized on 70S ribosomes.
当黑暗培养的眼虫细胞受到光照时，浓度为0.1 - 100微摩尔的放线菌酮刺激叶绿素合成。氯霉素（1 - 4毫摩尔）抑制叶绿素合成。放线菌酮对[¹⁴C]亮氨酸掺入三氯乙酸不溶物的影响，以及在短时间孵育中它未能影响[³²P]正磷酸盐掺入此类物质，被解释为放线菌酮特异性抑制80S核糖体蛋白质合成的证据。由于氯霉素对叶绿素合成的抑制作用被放线菌酮的存在所抵消，因此表明叶绿素合成受80S核糖体上合成的细胞质阻遏物控制，以及受70S核糖体上合成的去阻遏物控制。
