tyrP-乳糖操纵子融合菌株的构建及其在分离和分析tyrP表达去阻遏突变体中的应用。
Construction of a tyrP-lac operon fusion strain and its use in the isolation and analysis of mutants derepressed for tyrP expression.
作者信息
Kasian P A, Pittard J
出版信息
J Bacteriol. 1984 Oct;160(1):175-83. doi: 10.1128/jb.160.1.175-183.1984.
Abstract
The gene tyrP, which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. A tyrP-lac operon fusion was constructed and used to isolate mutants that have altered expression from the tyrP promoter. All putative tyrP operator mutations were transferred onto a plasmid vector by recombination in vivo. Restriction enzyme analysis of the resultant plasmids suggests that some of these mutants arose from either an insertion or a deletion of DNA occurring within the region of DNA that contains the tyrP promoter.
摘要
编码酪氨酸特异性转运系统一个组分的基因tyrP，已定位在大肠杆菌K-12染色体上42分钟处。构建了tyrP-lac操纵子融合体，并用于分离那些tyrP启动子表达发生改变的突变体。所有假定的tyrP操纵基因突变体通过体内重组转移到一个质粒载体上。对所得质粒的限制性酶切分析表明，这些突变体中的一些是由于在包含tyrP启动子的DNA区域内发生了DNA插入或缺失所致。
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