tyrP-乳糖操纵子融合菌株的构建及其在分离和分析tyrP表达去阻遏突变体中的应用。
Construction of a tyrP-lac operon fusion strain and its use in the isolation and analysis of mutants derepressed for tyrP expression.
作者信息
Kasian P A, Pittard J
出版信息
J Bacteriol. 1984 Oct;160(1):175-83. doi: 10.1128/jb.160.1.175-183.1984.
Abstract
The gene tyrP, which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. A tyrP-lac operon fusion was constructed and used to isolate mutants that have altered expression from the tyrP promoter. All putative tyrP operator mutations were transferred onto a plasmid vector by recombination in vivo. Restriction enzyme analysis of the resultant plasmids suggests that some of these mutants arose from either an insertion or a deletion of DNA occurring within the region of DNA that contains the tyrP promoter.
摘要
编码酪氨酸特异性转运系统一个组分的基因tyrP，已定位在大肠杆菌K-12染色体上42分钟处。构建了tyrP-lac操纵子融合体，并用于分离那些tyrP启动子表达发生改变的突变体。所有假定的tyrP操纵基因突变体通过体内重组转移到一个质粒载体上。对所得质粒的限制性酶切分析表明，这些突变体中的一些是由于在包含tyrP启动子的DNA区域内发生了DNA插入或缺失所致。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
[The biosynthesis of beta-galactosidase (lactase) in Escherichia coli; the specificity of induction].
Biochim Biophys Acta. 1951 Nov;7(4):585-99. doi: 10.1016/0006-3002(51)90072-8.
3
EFFECT OF INTEGRATED SEX FACTOR ON TRANSDUCTION OF CHROMOSOMAL GENES IN ESCHERICHIA COLI.
J Bacteriol. 1965 Mar;89(3):680-6. doi: 10.1128/jb.89.3.680-686.1965.
4
Selection for loss of tetracycline resistance by Escherichia coli.
J Bacteriol. 1981 Feb;145(2):1110-1. doi: 10.1128/jb.145.2.1110-1111.1981.
5
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
J Mol Biol. 1980 Apr;138(2):179-207. doi: 10.1016/0022-2836(80)90283-1.
6
Isolation and characterization of an Escherichia coli K-12 mutant defective in tyrosine- and phenylalanine-specific transport systems.
J Bacteriol. 1980 Jul;143(1):1-7. doi: 10.1128/jb.143.1.1-7.1980.
7
Model for regulation of the histidine operon of Salmonella.
Proc Natl Acad Sci U S A. 1980 Jan;77(1):508-12. doi: 10.1073/pnas.77.1.508.
8
Regulation of aromatic amino acid biosynthesis in Escherichia coli K-12: control of the aroF-tyrA operon in the absence of repression control.
J Bacteriol. 1980 Aug;143(2):613-20. doi: 10.1128/jb.143.2.613-620.1980.
9
Cloning and characterization of Escherichia coli K-12 regulator gene tyrR.
J Bacteriol. 1982 Dec;152(3):1276-9. doi: 10.1128/jb.152.3.1276-1279.1982.
10
Linkage map of Escherichia coli K-12, edition 7.
Microbiol Rev. 1983 Jun;47(2):180-230. doi: 10.1128/mr.47.2.180-230.1983.
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