反硝化菌株T1在厌氧利用甲苯过程中的底物诱导和代谢物积累
Substrate induction and metabolite accumulation during anaerobic toluene utilization by the denitrifying strain T1.
作者信息
Frazer A C, Ling W, Young L Y
机构信息
Institute of Environmental Medicine, New York University Medical Center, New York 10016.
出版信息
Appl Environ Microbiol. 1993 Sep;59(9):3157-60. doi: 10.1128/aem.59.9.3157-3160.1993.
Abstract
The denitrifying strain T1 utilizes toluene anaerobically. We now report that anaerobic toluene degradation is inducible in strain T1. Fluoracetate treatment of cell suspensions inhibited both the rate of toluene metabolism and the formation of the toluene dead-end products benzylsuccinate and benzylfumarate, which is consistent with the pathway proposed by Evans et al. (Appl. Environ. Microbiol. 58:496-501, 1992). In addition, when either nitrate was limiting or fluoroacetate was added, benzoate was detected during toluene metabolism.
摘要
反硝化菌株T1能在厌氧条件下利用甲苯。我们现在报告，在菌株T1中厌氧甲苯降解是可诱导的。用氟乙酸处理细胞悬浮液会抑制甲苯代谢速率以及甲苯末端产物琥珀酸苄酯和富马酸苄酯的形成，这与Evans等人（《应用与环境微生物学》58:496 - 501, 1992）提出的途径一致。此外，当硝酸盐受到限制或添加氟乙酸时，在甲苯代谢过程中会检测到苯甲酸。
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