Klein M, Hoffmann L
Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, Dummerstorf-Rostock, Germany.
Arch Tierernahr. 1993;44(2):95-109. doi: 10.1080/17450399309386061.
Male Wistar rats were fed in the live weight range between 70 and 200 g in periods alternately for maintenance and weight gain (semi ad libitum). The diets contained 10, 40 and 70% crude protein, resp., the ambient temperature was 30 degrees C. In agreement with two previous experiments 2 different feed regimes were used. Whereas in the first growth phase (up to about 150 g live weight) the supply of protein was constant for each group, in the second growth phase the protein level was changed between the groups from one period to the other. The energy metabolism on the feeding level of maintenance was measured by indirect calorimetry. At both feeding regimes a dependence of energy maintenance requirement on the protein level of feeding was established. The differences in the maintenance requirement between the 10% and 70% protein level and in the first growth phase also between the 10% and 40% protein level corresponded on average to a growth phase the expected values of which result from the different efficiency of ATP synthesis in the oxidative degradation of nutrients. It follows that--in accordance with the previous findings--the feeding regime does not influence the ratio of energetic utilization of nutrients in the maintenance metabolism of growing rats under the experimental conditions used by us. Further the results of all our studies on the dependence of the energy maintenance requirement of growing rats on the nutrient composition are summarized. We conclude that a dependence on the protein-carbohydrate ratio of the diet according to the different efficiency of ATP synthesis in the protein and carbohydrate catabolism can be reproducibly established by our experimental methods only then, if more than 50% of the energy maintenance requirement are covered from protein energy.
雄性Wistar大鼠在体重70至200克的范围内交替进行维持期和增重期喂养（半随意进食）。日粮中粗蛋白含量分别为10%、40%和70%，环境温度为30摄氏度。与之前的两项实验一致，采用了两种不同的喂养方式。在第一个生长阶段（直至体重约150克），每组的蛋白质供应保持恒定，而在第二个生长阶段，各实验组之间的蛋白质水平在不同时期发生变化。通过间接测热法测量维持喂养水平下的能量代谢。在两种喂养方式下，均确定了能量维持需求对日粮蛋白质水平的依赖性。10%和70%蛋白质水平之间以及第一个生长阶段10%和40%蛋白质水平之间维持需求的差异，平均相当于一个生长阶段，其预期值源于营养物质氧化降解中ATP合成的不同效率。由此可见，根据我们之前的研究结果，在所采用的实验条件下，喂养方式不会影响生长大鼠维持代谢中营养物质的能量利用比例。此外，我们总结了所有关于生长大鼠能量维持需求对营养成分依赖性的研究结果。我们得出结论，只有当日粮中超过50%的能量维持需求由蛋白质能量提供时，根据蛋白质和碳水化合物分解代谢中ATP合成的不同效率，日粮蛋白质 - 碳水化合物比例的依赖性才能通过我们的实验方法得到重现。
