Matthiessen M E, Romert P
Cell Tissue Res. 1976 Jun 14;169(2):179-92. doi: 10.1007/BF00214207.
Electron microscopic investigations of secretory ameloblasts from deciduous tooth germs of mini-pig foetuses and investigations of the ability of various fixatives to preserve these cells in tooth germs immersion-fixed in to 5 min, 10 min, 15 min, 20 min and 40 min after death of the mother gave the following results: 1. The ameloblasts exhibit ultrastructural characteristics typical of exocrine secretory cells of merocrine type. 2. The localization of organelles is as in rodent secretory ameloblasts, but differs from the location in the human analogues. 3. Fixation with 4% formaldehyde invariably gives unacceptable ameloblast preservation. 4. Fixation with 2.5% glutaraldehyde gives fair preservation of the ameloblasts when the germs are fixed within 10 min of the death of the mother. 5. Fixation with a fixative mixture 2% formaldehyde-1.25% glutaraldehyde gives good preservation when the ameloblasts are fixed within 15 min of the death of the mother. 6. Fixation with a fixative mixture 2% formaldehyde-1% glutaraldehyde-1% acrolein gives good ameloblast preservation when the germs are fixed within 15 min of the death of the mother.
对小型猪胎儿乳牙胚分泌性成釉细胞进行电子显微镜研究，并对各种固定剂在母体死亡后5分钟、10分钟、15分钟、20分钟和40分钟浸入固定的牙胚中保存这些细胞的能力进行研究，结果如下：1. 成釉细胞表现出典型的局部分泌型外分泌细胞的超微结构特征。2. 细胞器的定位与啮齿动物分泌性成釉细胞中的定位相同，但与人类类似细胞中的位置不同。3. 用4%甲醛固定总是导致成釉细胞保存效果不佳。4. 当牙胚在母体死亡后10分钟内固定时，用2.5%戊二醛固定能使成釉细胞得到较好的保存。5. 当成釉细胞在母体死亡后15分钟内固定时，用2%甲醛-1.25%戊二醛固定剂混合物固定能得到良好的保存效果。6. 当成釉细胞在母体死亡后15分钟内固定时，用2%甲醛-1%戊二醛-1%丙烯醛固定剂混合物固定能使成釉细胞得到良好的保存效果。
