一种来自漏斗网蜘蛛毒液的新型肽，ω-阿加毒素-TK，可选择性阻断P型钙通道。
A novel peptide from funnel web spider venom, omega-Aga-TK, selectively blocks, P-type calcium channels.
作者信息
Teramoto T, Kuwada M, Niidome T, Sawada K, Nishizawa Y, Katayama K
机构信息
Eisai Tsukuba Research Laboratories, Ibaraki, Japan.
出版信息
Biochem Biophys Res Commun. 1993 Oct 15;196(1):134-40. doi: 10.1006/bbrc.1993.2225.
In the course of purification of omega-Aga-IVA, a specific P-type calcium channel blocker, from the venom of Agelenopsis aperta we discovered a novel peptide. This peptide, named omega-agatoxin Tsukuba (omega-Aga-TK), also blocked P-type channels and was twelve times more abundant in the venom than omega-Aga-IVA. omega-Aga-TK was purified to homogeneity by a two-step reverse-phase HPLC procedure. Its amino acid sequence is 71% identical to that of omega-Aga-IVA. omega-Aga-TK has a negatively charged N-terminus, whereas omega-Aga-IVA has a positively charged one. Electrophysiological data indicate that omega-Aga-TK is a potent and selective inhibitor of P-type channels.
在从墨西哥漏斗蛛毒液中纯化一种特定的P型钙通道阻滞剂ω-Aga-IVA的过程中，我们发现了一种新的肽。这种肽名为筑波ω-阿加毒素（ω-Aga-TK），也能阻断P型通道，并且在毒液中的含量是ω-Aga-IVA的12倍。通过两步反相高效液相色谱法将ω-Aga-TK纯化至同质。其氨基酸序列与ω-Aga-IVA的氨基酸序列有71%的同一性。ω-Aga-TK的N端带负电荷，而ω-Aga-IVA的N端带正电荷。电生理数据表明，ω-Aga-TK是P型通道的一种强效且选择性的抑制剂。
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