Camello P J, Salido G M
Department of Physiology, Faculty of Veterinary Science, University of Extremadura, Cáceres, Spain.
Biochem Pharmacol. 1993 Sep 14;46(6):1005-9. doi: 10.1016/0006-2952(93)90664-i.
In many tissues the cellular responses mediated through different intracellular messenger systems are mutually interactive. In the exocrine pancreas the secretagogues acting via adenosine cyclic monophosphate (cAMP) and those acting via calcium-phosphoinositides can potentiate one another. On the other hand, protein kinase C (PK-C) modulates receptor-induced responses in exocrine pancreatic cells and other cell types. Recording total protein output, monitored on-line at 280 nm, from superfused rat pancreatic segments, we demonstrate that secretin (a cAMP-acting hormone) reduces the efficacy of the calcium-mediated secretagogue cholecystokinin-octapeptide (CCK-8). Likewise, the PK-C activator 12,O,tetradecanoyl phorbol 13 acetate (TPA) reduces both the efficacy of secretin and the potency of cholecystokinin. Thus, the hypothesis of potentiation between different stimulus-secretion coupling mechanisms must be revised, and receptor-activated responses in the exocrine pancreas must be considered a complex model with multiple inhibitory and stimulatory interactions.
在许多组织中，通过不同细胞内信使系统介导的细胞反应是相互作用的。在外分泌胰腺中，通过环磷酸腺苷（cAMP）起作用的促分泌剂和通过钙 - 磷酸肌醇起作用的促分泌剂可以相互增强作用。另一方面，蛋白激酶C（PK - C）调节外分泌胰腺细胞和其他细胞类型中受体诱导的反应。通过记录从灌注的大鼠胰腺段在线监测的280nm处的总蛋白输出，我们证明促胰液素（一种作用于cAMP的激素）降低了钙介导的促分泌剂八肽胆囊收缩素（CCK - 8）的效力。同样，PK - C激活剂12 - O - 十四烷酰佛波醇13 - 乙酸酯（TPA）降低了促胰液素的效力和胆囊收缩素的效能。因此，必须修正不同刺激 - 分泌偶联机制之间增强作用的假设，并且外分泌胰腺中受体激活的反应必须被视为具有多种抑制和刺激相互作用的复杂模型。
